Development and testing of a bacterial biosensor for toluene-based environmental contaminants

Article Abstract:

A bacterial biosensor for benzene, toluene and similar compounds appears to be capable of accurately measuring environmental contaminants, making it a potentially inexpensive tool for field-ready assays. A reporter plasmid was constructed by placing the luc gene for firefyl luciferase directly under the control of the P(sub u) promoter and the xylR transcriptional activator of Pseudomonas putida mt-2. The biosensor cells were shown to detect benzene, toluene and xylene contamination in soil and water samples with high sensitivity and quantitative accuracy.

Analysis, Evaluation, Biosensors, Pollutants

---

Community analysis of biofilters using fluorescence in situ hybridization including a new probe for the Xanthomonas branch of the class Proteobacteria

Article Abstract:

A 16S rRNA-targeted oligonucleotide probe is described that can hybridize with the Xanthomonas branch of the Proteobacteria. These bacteria are used as biofilters to filter waste gases from air during industrial processes.

Usage, Bacteria, In situ hybridization, Identification and classification

---

Similar abstracts:

• Abstracts: Suppression of bacterial blight by a bacterial community isolated from the guttation fluids of anthuriums. Degradation of phenanthrene, fluorene, fluoranthene, and pyrene by a mycobacterium sp
• Abstracts: Biology of DNA restriction. Gliding motility in bacteria: insights from the studies of Myxococcus xanthus. Recent advances in the social and developmental biology of the myxobacteria
• Abstracts: Microbial production of surfactants and their commercial potential. P(sub)II signal transduction proteins, pivotal players in microbial nitrogen control
• Abstracts: Overexpression of an agouti cDNA in the skin of transgenic mice recapitulates dominant coat color phenotypes of spontaneous mutants
• Abstracts: Rickettsia rickettsii infection of the EA.hy 926 endothelial cell line: morphological response to infection and evidence for oxidative injury

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.