Survival of Vibrio parahaemolyticus at low temperatures under starvation conditions and subsequent resuscitation of viable, nonculturable cells

Article Abstract:

Cells of Vibrio parahaemolyticus subjected to starvation at low temperature grow and multiply at high rates with a rise in temperature and limited nutrient conditions. The shape of the cells changes from a rod shape to a spherical shape when the culture is subjected to starvation under different temperatures. Nonculturable stages are reached under starvation at 3.5 degrees Celsius in 50 to 80 days. The Kanagawa (K)-positive strains lose culturability faster than the K-negative cells. Low temperatures appear to affect the cells more than starvation.

Physiological aspects, Cells (Biology), Growth, Cells, Low temperatures

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Effect of bile in Vibrio parahaemolyticus

Article Abstract:

The addition of bile or the bile acid deoxycholic acid to estuarine water-cultured bacteria was found to cause an increase in the direct viable count and colony counts among the virulent strains of the enteric pathogen Vibrio parahaemolyticus. This effect was not observed in the nonvirulent strains and was reversed by extraction of bile acids with cholestyramine. Bile-treated V. parahaeomolyticus had lower levels of intracellular calcium than untreated cells.

Bile acids

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Effect of bile on Vibrio parahaemolyticus

Article Abstract:

A study was conducted to determine if the Vibrio parahaemolyticus, an enteric pathogen found in estuarine water, is affected by bile or its deoxycholic acid component. Results show that the addition of bile led to a rise in the colony counts or direct viable count among the virulent strains. Moreover, bile acids enhance the expression of virulence factors. This study confirmed the important role of bile in bacterial regulation.

Deoxycholic acid

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