Heterogeneity of iron bioavailability on plants assessed with a whole-cell GFP-based bacterial biosensor

Article Abstract:

Heterogeneity of iron bioavailability is discussed based on study in which plants were assessed with a whole-cell green fluorescent protein (GFP)-based bacterial biosensor. Knowledge of variability in iron bioavailability to individuals is required to assess the nature of iron competition in natural habitats. Pseudomonas syringae ferric iron availability was evaluated by measuring the fluorescence intensity of single cells which have a plasmid-borne transcriptional fusion of an iron-regulated promoter from a locus that encodes a membrane receptor for a pyoverdine siderophore with a reporter gene encoding GFP after fluorescence microscopy.

United States, Statistical Data Included, Usage, Measurement, Genetic aspects, Bacteria, Biosensors, In situ hybridization, Fluorescence, Pseudomonas syringae

---

Survival of, and induced stress resistance in, carbon-starved Pseudomonas fluorescens cells residing in soil

Article Abstract:

Exponentially growing carbon-starved Pseudomonas fluorescens cells develop resistance to lethal conditions such as ethanol exposure and high temperature when placed in different soils. The carbon-starved P. fluorescens cells placed in Ede loamy sand show a decrease in the inoculant cell and CFU count and an increase in nonculturability, as compared to those placed in Flevo silt loam. The decrease in cell length is the same in both soils. Glucose increases the survival of the bacteria and prevents the decrease in cell size.

Research, Analysis, Stress (Physiology), Carbon, Soil research, Pseudomonas fluorescens

---

Similar abstracts:

• Abstracts: The viable-but-nonculturable state induced by abiotic stress in the biocontrol agent Pseudomonas fluorescens CHA0 does not promote strain persistence in soil
• Abstracts: Detection of N-acylhomoserine lactones in lung tissues of mice infected with Pseudomonas aeruginosa. Quantification of biofilm structures by the novel computer program COMSTAT
• Abstracts: Regulatory interactions between the Hrp type III protein secretion system and coronatine biosynthesis in Pseudomonas syringae pv. tomato DC3000
• Abstracts: Comparison of toxinotyping and PCR ribotyping of Clostridium difficile strains and description of novel toxinotypes
• Abstracts: The biocontrol strain Pseudomonas fluorescens F113 produces the Rhizobium small bacteriocin, N-(3-hydroxy-7-cis-tetradecenoyl)homoserine lactone, via HdtS, a putative novel N-acylhomoserine lactone synthase

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.