Synthesis and selection of de novo proteins that bind and impede cellular functions of an essential mycobacterial protein

Article Abstract:

The synthesis and selection of codon-shuffled de novo proteins that bind to a selected mycobacterium tuberculosis protein target, the histone-like protein HupB, believed to be essential for mycobacterial growth is reported. The observations indicate that the expression of the de novo protein binder in Mycobacterium smegmatis causes a reduction of growth of the recombinant strain in the exponential phase, a decrease in the transcription levels of the hubB gene, a decrease in the overall colony count, a reduction in the capacity of the recombinant strain to withstand cold shock stress and changes in cell morphology.

Analysis, Protein synthesis, RNA, Protein biosynthesis, Bacterial proteins, Properties, RNA synthesis

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Root-associated bacteria contribute to mineral weathering and to mineral nutrition in trees: A budgeting analysis

Article Abstract:

Two column experiments with a quartz-biotite substrate were conducted to quantify the respective effects of plant- and root-associated bacteria on mineral weathering and their consequences on tree seedling growth and nutrition. It was observed that there was no improvement in growth when the seedlings were supplied with all the necessary nutrients suggesting that the principal nutrient source for forest trees is derived from the weathering of soil minerals, which results from water circulation and from plant and microbial activity.

Plant ecophysiology, Seedlings, Plant physiological ecology

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Metschnikowia strains isolated from botrytized grapes antagonize fungal and bacterial growth by iron depletion

Article Abstract:

Pigment-producing Metschnikowia strains were isolated from noble-rotted grapes that had antagonistic activity against filamentous fungi, yeast and bacteria. It was analyzed that the proposed mechanism was different from the mechanisms operating in microbes that released siderophores into the environment for iron acquisitions, as the pigment was a large nondiffusible complex produced in the presence of both low and high concentrations of ferric ions.

Hungary, Citrus fruits, Citrus (except Orange) Groves, Grapefruit, Physiological aspects, Insect resistance (Plants), Grapefruits

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