System using tandem repeats of the cA peptidoglycan-binding domain from Lactococcus lactis for display of both N- and C-terminal fusions on cell surfaces of lactic acid bacteria

Article Abstract:

A system is established for displaying heterologous protein to the C terminus of the peptidoglycan-binding domain (cA domain) of a major autolysin from Lactococcus lactis (AcmA). The cell surface has shown heterologous protein on the C terminus of the cA domain by using AmyA as a model protein and an increase in binding capacity by using tandem repeats of the cA domain.

Environmental aspects, Proteins, Peptidoglycans, Protein structure, Lactococcus

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Display of alfa-amylase on the surface of Lactobacillus casei cells by use of the PgsA anchor protein, and production of lactic acid from starch

Article Abstract:

A study was made on the fusion of the N terminus of the target protein with the PgsA protein by constructing a novel starch-degrading strain of Lactobacillus casei. It was observed that the recombinant L. casei cells with cell surface display of AmyA helped in the efficient simultaneous saccharification and fermentation from soluble starch to lactic acid.

Usage, Fermentation, Fluorescence microscopy, Bacterial proteins, Properties

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Functional analysis of the fructooligosaccharide utilization operon in Lactobacillus paracasei 1195

Article Abstract:

The cell fractionation experiments which show the fructooligosaccharide (FOS) hydrolysis activity present exclusively in the cell wall extract of Lactobacillus paracasei grown on FOS is reported.

Analysis, Hydrolysis, Gastrointestinal system, Cell fractionation

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