Systematic sequencing of the Bacillus subtilis genome: progress report of the Japanese group

Article Abstract:

The Japanese Human Genome Program forms a part of the international co-operative project between Japan and the European Community for the systemic sequencing of the Bacillus subtilis genome. Regions that include 700 kb have been cloned till Feb 1995 using inverse PCR technique. Nearly 500 kb has been sequenced in steps, each of which sequences a lambda insert of 10-15 kb in 2 to 3 weeks. The sequences analyzed encode 437 putative open reading frames in total. Some of the new features identified include genes associated with macromolecular synthesis, two aminoacyl-tRNA synthesis genes and the primary structures of hut, pts, iol and nar-nir operons.

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Nucleotide sequence and analysis of the phoB-rrnE-groESL region of the Bacillus subtilis chromosome

Article Abstract:

A 36 kb sequence of the phoB-rrnE-groESL region of the Bacillus subtilis chromosome at around 55 degrees was analyzed to determine the gene organization of the region. Results show that the region contained 36 ORFs and the whole rrnE operon. Most of these ORFs were transcribed in the same direction as the chromosome replication while the phoB gene was transcribed in the opposite direction. The sequenced region also contained an operon consisting of genes for degradation and uptake of mannan.

Research, Genomes, Nucleotides

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Determination of a 17484 bp nucleotide sequence around the 39 degree region of the Bacillus subtilis chromosome and similarity analysis of the products of putative ORFs

Article Abstract:

A 17484 bp sequence of the 39 degree region of the Bacillus subtilis chromosome contains open reading frames (ORFs), and the restriction sites BamHI, EcoRI and PstI. There are 17 ORFs which have an initiation codon of ATG, GTG or TTG. The transcription and translation of all ORFs is in the same direction. The proteins encoded by the ORFs and their similarity to other proteins are discussed.

Similar abstracts:

• Abstracts: Organization of the European Bacillus subtilis genome sequencing project. SubtiList: a relational database for the Bacillus subtilis genome

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