Teschoviruses as indicators of porcine fecal contamination of surface water

Article Abstract:

Research describes the development of a real-time reverse transcriptase coupled polymerase chain reaction method for the quantitative detection of pig teschovirus RNA. in contaminated water. Data show that the method is very specific, sensitive and detects 92 femtogram of viral RnA in one milliliter of water sample. Further, the method identifies the origin of the contamination unlike other methods.

Spain, Meat industry, Water, Statistics, Statistics (Data), RNA, Contamination

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Multiplex reverse transcription-PCR for simultaneous detection of Beet necrotic yellow vein virus, Beet soilborne virus and Beet virus Q and their vector polymyxa betae KESKIN on sugar beet

Article Abstract:

The detection threshold pf the multiplex reverse transcription-polymerase chain reaction assay for the three title viruses and their vector is up to 128 times greater than that of an enzyme-linked immunosorbent assay. Data indicate that Beet necrotic yellow vein virus is associated with one or two pomoviruses.

Belgium, Pesticide and Other Agricultural Chemical Manufacturing, Agricultural chemicals, not elsewhere classified, Turkey, Sugarcane and sugar beets, Sugar Beets, Sugar Beet Farming, Sugarbeet Herbicides, Diseases, Plant viruses, Sugar beet, Virus diseases of plants

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Multiplex quantitative real-time reverse transcriptase PCR for [F.sup.+]-specific RNA coliphages: A method for use in microbial source tracking

Article Abstract:

A culture-independent multiplex real-time reverse transcriptase-PCR (RT-PCR) protocol for the simultaneous quantification of all four subgroups of [F.sup.+]-specific RNA coliphages using novel primer sets and molecular beacons is presented.

Bacteriophage T4

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