The Bacillus stearothermophilus NUB36 surA gene encodes a thermophilic sucrase related to Bacillus subtilis SacA

Article Abstract:

The sucrase, encoded by the surA gene of Bacillus stearothermophilus, shows a sequence similarity with sucrose hydrolising enzymes of B. subtilis, Klebsiella pneumoniae and Vibrio alginolyticus. The enzyme's sucrose box, a sequence of nine amino acids, is important in the catalysis of sucrose into glucose and fructose. SurA shows a high degree of stability at room temperature, with an optimum temperature of 55 degree celsius for sucrose hydrolysis. The active form of the enzyme is a dimer and it is unable to operate during sucrose phosphate cleavage.

Genetic aspects, Bacillus (Bacteria), Bacillus, Enzymatic analysis

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bctA: a novel pBF4 gene necessary for conjugal transfer in Bacteroides spp

Article Abstract:

The genetic basis of pBF-4-mediated conjugal transfer in Bacteroides spp. was investigated. Recombinatorial mutagenesis using a suicide vector carrying fragments of the pBF4 plasmid was used to identify pBF4 genes governing conjugation. The identification of the first conjugation-specific gene of pBF4 was foreseen to provide the basis for further investigations of pBF4-mediated conjugation.

Usage, Mutagenesis, Conjugation (Biology), Conjugation (Reproduction)

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