The Bifidobacterium longum NCIMB 702259(super T) ctr gene codes for a novel cholate transporter

Article Abstract:

Pre-exposure of Bifidobacterium longum NCIMB 702259(super T) to cholate caused increased resistance to cholate, chloramphenicol, and erythromycin. The B. longum ctr gene, encoding a cholate efflux transporter, was transformed into the efflux-negative mutant Escherichia coli KAM3, conferring resistance to bile salts and other antimicrobial compounds and causing the efflux of [(super 14)C] cholate.

Usage, Nucleotide sequence, Base sequence, Phylogeny, Properties, Chloramphenicol

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Identification of N-acetylhexosamine 1-Kinase in the complete lacto-N-biose I/galacto-N-biose metabolic pathway in Bifidobacterium longum

Article Abstract:

The functions of the enzymes encoded by the lnpB, lnpC, and lnpD genes, located downstream of the lacto-N-biose phosphorylase gene (lnpA) are determined in Bifidobacterium longum JCM1217. The results of the study suggest that the gene operon InpABCD encodes an undescribed lacto-N-biose I/galacto-N-biose metabolic pathway.

Cell metabolism

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Novel putative galactose operon involving lacto-N-biose phosphorylase in Bifidobacterium longum

Article Abstract:

A lacto-N-biose phosphorylase (LNBP) was purified from the cell extract of Bifidobacterium bifidum JCM1254 and a gene from the strain of B. longum JCM1217 was successfully cloned. The enzyme was not homologous with any other known protein and that LNBP is the bifidus factor.

Phosphorylase

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