The cyro-EM structure of a translation initiation complex from Escherichia coli
Article Abstract:
The 70S ribosome and its complement of factors required for initiation of translating in E. coil were purified separately and reassembled in vitro with GDPNP producing a stable initiation complex (IC) stalled after 70S assembly. A cryo-EM reconstruction of the IC showing IF2.GDPNP at the intersubunit cleft of the 70S ribosome is obtained.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2005
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Nascent ribosomes
Article Abstract:
This review examines recent understanding about the ribosomal RNA transcript conversion to a nascent cytoplasmic ribosome. Data suggest that ribosomal RNA processing involves a large number of accessory RNA and protein factors, their assembly and finally their export to the cytoplasm.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2001
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