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The gene encoding IIAB(super Man)(sub L) in Streptococcus salivarius is part of a tetracistronic operon encoding a phosphoenolpyruvate:mannose/glucose phosphotransferase system

Article Abstract:

The man operon that codes the components of the phosphoenolpyruvate:mannose phosphotransferase system (PTS) in Streptococcus salivarius is described. This system is responsible for the transport of glucose and mannose.

Author: Lortie, Louis-Andre, Pelletier, Michel, Vadeboncoeur, Christian, Frenette, Michel
Publisher: Society for General Microbiology
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 2000
Biological transport, Active, Active biological transport, Microbial metabolism, Glucose metabolism

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Genetic and biochemical characterization of the phosphoenolpyruvate:glucose/mannose phosphotransferase system of Streptococcus thermophilis

Article Abstract:

Research has been conducted on Streptococcus thermophilis ATCC 19258 operons. The authors discuss their characterization, overproduction and purification carried out via in vitro phosphoenolpyruvate-dependent phosphorylation experiments using phosphotransferase system purified proteins.

Author: Vadeboncoeur, Christian, Frenette, Michel, Moineau, Sylvain, Cochu, Armelle
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
Canada, Biochemistry, Research and Development in the Physical, Engineering, and Life Sciences, Science & research, Physiological aspects, Environmental aspects, Microbiology, Bacterial proteins, Microbial populations, Glucose, Dextrose, Phosphotransferases, Microbial ecology

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An effective strategy, applicable to Streptococcus salivarius and related bacteria, to enhance or confer electroporation competence

Article Abstract:

A straightforward, reproducible method for generating high-efficiency, electroporation-competent S. salivarius is described. The protocol involves glycine treatment of cells growing in rich medium, followed by electroporation. The approach was used to introduce a plasmid into other strains of S. salivarius including a fresh isolate. The method was successfully used with other streptococci and related bacteria.

Author: Vadeboncoeur, Christian, Frenette, Michel, Buckley, Nicole D., LeBlanc, Donald J., Lee, Linda N.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
Bacteria, Bacterial transformation

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Subjects list: Research, Genetic aspects, Streptococcus
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