The phagosome containing Legionella pneumophila within the protozoan Hartmannella vermiformis is surrounded by the rough endoplasmic reticulum

Article Abstract:

The capacity of Legionella pneumophila to reside and replicate within Hartmannella vermiformis and produce infection depends on being surrounded by the rough endoplasmic reticulum (RER). RER is present around most of the phagosomes with L. pneumophila between 5 and 15 hrs after infection but absent after 20 hrs. The endoplasmic reticulum-specific protein (BiP) is present in large amounts in phagosomes of L. pneumophila 5 hrs after infection. Ribosomes, mitochondria and the BiP protein are, however, absent in the phagosomes after 20 hrs of infection.

Endoplasmic reticulum, Bacterial infections

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Signal transduction in the protozoan host Hartmannella vermiformis upon attachment and invasion by Legionella micdadei

Article Abstract:

Research was conducted to examine the initial signaling events in the protozoan host Hartmannella vermiformis upon attachment to and invasion by the Legionnaires' disease bacterium L. micdadei. Focus was on the influence of the Gal/GalNAc lectin in the attachment and invasion by Legionella micdadei. Results reveal similarities in the L. pneumophila and L. micdadei receptor-mediated signaling events in H. vermiformis. However, the two species are targeted into different compartments in the protozoan host.

Cellular signal transduction, Protozoa, Protozoans

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Gentamicin-containing peptone-yeast extract medium for cocultivation of Hartmannella vermiformis ATCC 50256 and virulent strains of Legionella pneumophila

Article Abstract:

Gentamicin-containing peptone-yeast (PY) medium in cocultivation with Hartmannella vermiformis ATCC 50256 helps in differentiation of virulent and avirulent Legionella pneumophila strains. A virulent strain of L. pneumophila and its avirulent derivative multiply in cocultures using PY medium. Gentamicin however, decreases extracellular growth of the avirulent strain of L. pneumophila in cocultures and allows only the virulent strain to multiply.

Culture media (Biology), Culture media, Gentamicin, Gentamicins

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