The presence of two S-layer-protein-encoding genes is conserved among species related to Lactobacillus acidophilus

Article Abstract:

The existence of S-protein and the corresponding S-protein-encoding genes of strains belonging to species that are closely related to Lactobacillus acidophilus, was investigated. Strains of L. acidophilus, L. crispatus, L. amylovorus and L. gallinarum have an S-layer and two slp genes. Strains of L. helveticus have an S-layer but have only one intact slp gene. Strains of L. gasseri, L. johnsonii and L. delbrueckii subsp. bulgaricus have neither an S-layer nor S-protein-encoding genes hybridizing with probes derived from the L. acidophilus slpA or slpB region.

Lactobacillus, Proteins

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Genetic analysis of acidocin B, a novel bacteriocin produced by Lactobacillus acidophilus

Article Abstract:

DNA sequence analysis of strain M46 of Lactobacillus acidophilus reveals the presence of three genes, one of which has an amino acid composition similar to acidocin B and is the gene that codes for acidocin B. The metabolic activity of lactic acid bacteria (LAB) plays an important role in its action to preserve food. The formation of metabolic products which are acidic creates a condition that is not favorable for bacterial growth. The bacteriocins produced by LAB fall into heat-stable, heat-labile, lantibiotic and complex bacteriocin categories.

Analysis, Amino acid sequence, Amino acid sequencing, Lactobacillus acidophilus

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A convenient and reproducible method to genetically transform bacteria of the genus Bifidobacterium

Article Abstract:

A system for the efficient and reproducible genetic transformation of strains of the genus Bifidobacterium is developed. The system, which allows foreign plasmid DNA to be introduced into Bifidobacterium, has already been tested on several Bifidobacterium species. It is based on the preincubation of the bacteria at low temperatures in electroporation buffer prior to electroporation and on the use of plasmid vectors with a replicon from Actinomycetaceae.

Bacterial genetics, Bifidobacterium

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