Toward a dog radiation hybrid map

Article Abstract:

A study was conducted to develop a dog genome map to be utilized for linkage studies and wide genome search and comparison. Dog fibroblasts were fused after gamma irradiation with thymidine kinase-deficient hamster cells. The dog fibroblasts were irradiated with 5,000 rad. Cell lines were then determined and prepared in roller bottles. In addition, individual clones were picked and prepared at 37 degrees C in microtiter plates.

Genomes, Chromosome mapping, Fibroblasts

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A survey of canine expressed sequence tags and a display of their annotations through a flexible Web-based interface

Article Abstract:

Research has been conducted on canine expressed sequence tags from different DNA libraries. The results of the sequence analysis of these tags have demonstrated that they provide sequence information for 5%-10% of the canine genes.

United States, Science & research, Research and Testing Services, Scientific Research and Development Services, Administration of Air and Water Resource and Solid Waste Management Programs, Science, Research & Development, Basic Research, Solid Waste R&D, Physiological aspects, Comparative analysis, Gene expression, Heredity, Methodology, Research methods, Genetic research, DNA sequencers

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