Transcription termination signals in the nin region of bacteriophage lambda: identification of rho-dependent termination regions

Article Abstract:

The roc region, a subregion of the nin region of bacteriophage lambda, shows transcription activity, and the terminators in the roc region are Rho dependent. There are many terminators in the two roc region segments, tR3 and tR4. Gene expression is controlled by transcription termination signals and the terminators function when translation and transcription are not coupled. Escherichia coli studies reveal two classes of transcription terminators, the intrinsic, which functions in an in vitro system without a transcription termination protein, and the Rho dependent, which functions as a hexamer causing termination of transcription.

Analysis, Genetic regulation, Genetic transcription, Transcription (Genetics)

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Short-patch reverse transcription in Escherichia coli

Article Abstract:

Bases connected to ribonucleotide backbone positions in chimeras can code DNA indicating that the in vivo DNA polymerases use a DNA backbone to continue into a backbone junction and then into ribonucleotide area to come back to a normal DNA backbone. RNA and DNA are associated as hybrids in which complementary RNA and DNA strands are paired by hydrogen bonds and chimeras in which both ribonucleotides and deoxyribonucleotides are present on a covalent backbone of phosphodiester. The reverse transcription that occurs in Escherichia coli may be termed as short-patch reverse transcription.

Research, Usage, Observations, Mosaicism, DNA polymerases

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Genetic recombination in bacteriophage t4: single-burst analysis of consegregants and evidence in favor of a splice/patch coupling model

Article Abstract:

T4 bacteriophage recombinant progeny were analyzed using a single-burst technique to determine the structure of the intermediate products of genetic recombination. The analysis was done in multifactor crosses to quantitatively measure the products of recombination. Results indicate that the principal recombinant structures are splice/patch pairs synchronously formed. From these results a model for recombination named splice/patch coupling model was developed.

Models, Genetic aspects, Genetic recombination, Bacteriophage T4

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