Transfer of transposon Tn916 from Bacillus subtilis into a natural soil population

Article Abstract:

Horizontal transfer of the Tn916 gene, which includes a gene that codes for tetracycline resistance in soil populations, was investigated using Bacillus subtilis as the vector. The percentage of tetracycline-resistant organisms in the experimental soils significantly increased, reaching up to 60% after the fourth day. All recipients were identified as actinomycetes, including three genera, namely, Streptomyces, Actinomadura and Kineosporia. These actinomycetes have not previously been reported to be Tn916 recipients.

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Temperature-dependent induction of an acid-inducible stimulon of Escherichia coli in broth

Article Abstract:

The induction of the lysyl-tRNA synthetase system or LysU, inducible lysine decarboxylase and inducible arginine decarboxylase is temperature-dependent in Escherichia coli when grown in broth to a pH of 5.5 or lower. Coordinate induction of these enzymes, as well as other peptides such as the heat-shock peptides HtpG and HtpM, suggest that these make up part of a stimulon which is induced in a temperature-dependent manner at low pH.

Microbial enzymes

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High-frequency phage-mediated gene transfer among Escherichia coli cells, determined at the single-cell level

Article Abstract:

Cycling primed in situ amplification-fluorescent in situ hybridization was employed to accurately determine the frequency of phage-mediated gene transfer. The results found that the differences in the number of viable cells carrying the transferred gene and the number of cells capable of growth on the selective medium are 3 to 4 orders of magnitude.

Science & research, Analysis, Growth, In situ hybridization, Bacterial cell walls, Company growth

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