Transition from nonspecific to specific DNA interactions along the substrate-recognition pathway of dam methyltransferase

Article Abstract:

The effects of substitutions in the related Escherichia coli DNA methyltransferase (EcoDam), altering residues corresponding to those involved in specific interaction with the canonical GATC target sequence in T4Dam is reported. The two types of protein-DNA interactions, namely, discriminatory contacts and anti-discriminatory contacts are detailed.

United States, Escherichia coli, Methyltransferases

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Transportation proteins and DNA uptake localize to the poles in Bacillus subtilis

Article Abstract:

Fluorescence microscopy is used to study the localization of several competence proteins, required for DNA uptake as the Gram-positive, rod-forming bacterium Bacillus subtilis is found to efficiently bind and internalize, transforming DNA. The results suggest the regulated assembly and disassembly of a DNA-uptake machine at the cell poles.

Usage, Genetic aspects, Bacillus subtilis, Fluorescence microscopy

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Crystal structures of RNase H bound to an RNA/DNA hydrid: Substrate specificity and metal-dependent catalysis

Article Abstract:

The crystal structures of RNase H complexed with an RNA/DNA hybrid and a mechanism for substrate recognition and two-metal-ion dependent catalysis are reported. RNase H specifically recognizes the A form RNA strand recognition and it can be concluded that two-metal-ion catalysis is a general feature of the superfamily.

RNA, Hybridization

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