Characterization of the 450-kb linear plasmid in a polychlorinated biphenyl degrader, Rhodococcus sp. strain RHA1

Article Abstract:

Researchers describe the pRHL2 plasmid of Rhodococcus sp. strain RHA1. This plasmid contains genes for biodegrading polychlorinated biphenyls.

Genetic aspects, Plasmids

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Multiple polychlorinated biphenyl transformation systems in the gram-positive bacterium Rhodococcus sp. strain RHA1

Article Abstract:

Rhodococcus erythropolis IAM1399 cells expressing the bphA gene of Rhodococcus sp. strain RHA1 are capable of degrading di-, tri-, and tetrachlorobiphenyls. Mutation of the bphA1 gene suppresses bacterial growth on biphenyl and polychlorinated biphenyl (PCB) transformation activity. The bphA1 insertion mutant RDA1 is able to transform PCB congeners when cultured on ethylbenzene as its carbon source. The mutant is also able to convert 4-chlorobiphenyl to 4-chlorobenzoate suggesting the presence of a PCB degradation system different from that encoded by the bph genes.

Observations, Biphenyl compounds, Biphenyls (Class of compounds)

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Two nearly identical aromatic compound hydrolase genes in a strong polychlorinated biphenyl degrader, Rhodococcus sp. strain RHA1

Article Abstract:

A study was conducted to analyze the activities, structures and expression of genes encoding 2-hydroxy-6-oxohepta-2,4-dienoate and 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolases to determine the functions of the enzymes in the catabolism of aromatic compounds. A Kilo-sequence deletion equipment was utilized to prepare a series of deletion clones. Results showed that etbD1 gene product is involved in the meta-cleavage metabolic pathway of ethylbenzene.

Enzymes, Metabolism, Aromatic compounds

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