A novel positive selection for identifying cold-sensitive myosin II mutants in dictyostelium

Article Abstract:

Novel positive selection for myosin heavy chain mutants are generated in soil amoeba Dictyostelium. The selection assumed substrate release of wild-type cells and adherence of myosin null cells after minimal azide exposure. Mutants were localized to a domain encoded by the myosin gene. Some mutant sequences exhibited retention of cold-sensitive properties when expressed in cells without wild-type myosin.

Genetic aspects, Microbial mutation, Dictyostelium, Myosin

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Progeny of germ line knockouts of ASI2, a gene encoding a putative signal transduction receptor in Tetrahymena thermophila, fail to make the transition from sexual reproduction to vegetative growth

Article Abstract:

Analysis of role of cache domain as signal transduction receptor during replication of deoxyribonuceic acid in Tetrahymena thermophila is presented.

United States, Science & research, Biological Product (except Diagnostic) Manufacturing, Drugs, Deoxyribonucleic Acid, Analysis, DNA, Protein metabolism

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