Use of a microscope photometer to analyze in vivo fluorescence intensity of epilithic microalgae grown on artificial substrata

Article Abstract:

An epifluorescence microscope photometer was employed to establish a new, in vivo fluorimetric technique for analyzing fluorescence intensities of epilithic microalgae grown on clay tiles in the field. This permitted a nondestructive, direct quantification of algal biomass on the substratum surface. This in vivo analysis is applicable to ecological experiments and offers the benefit of measuring on an extremely small scale, which allows direct analysis of the microdistribution of epilithic microalgae in live biofilms.

Research, Equipment and supplies, Measurement, Algae, Fluorescence, Photometry

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Online tool for analysis of denaturing gradient gel electrophoresis profiles

Article Abstract:

An online tool is submitted which enumerate the matching of two groups which are deemed to be similar in the distinct samples and also in the situation when the models are used in the distinct denaturing gradient gel electrophoresis gels. Although the successive characteristics are not specified clearly, the presumption that the fragments are identical is based on the fact that the groups seem to be consistent in the models, which are selected from the same environment.

Models, Gel electrophoresis, Ribosomal RNA

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Use of fluorescent lectin probes for analysis of footprints from Pseudomonas aeruginosa MDC on hydrophilic and hydrophobic glass substrata

Article Abstract:

A clean or a sinalized glass was connected to the microbial footprints of Pseudomonas aeruginosa MDC for 1 h and were evaluated with the help of lectin probes that were fluorescently labeled. The substrata which was utilized for the formation of footprints was possible due to the hydrophilic glass slides that were clean or were made hydrophobic with the help of the salinzation technique of Neu and Marshall.

Usage, Lectins, Pseudomonas aeruginosa, Substrates

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