Use of an excision reporter plasmid to study the intercellular mobility of the conjugative transposon Tn916 in Gram-positive bacteria

Article Abstract:

An excision reporter plasmid pTCR9 was created to qualitatively and quantitatively characterize the intracellular mobility of the conjugative transposon Tn916 in various Gram-positive bacteria including isogenic strains with different donor potential. The excision event was suggested to be insufficient for conjugal transfer to occur and that there was no linear relationship between Enterococcus faecalis strains and either the excision frequency of pAT112 or the copy number of Tn916 circular intermediates per cell in the hosts.

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Escherichia coli ColV plasmid pRK100: genetic organization, stability and conjugal transfer

Article Abstract:

A ColV plasmid of a uropathogenic strain of Escherichia coli and its host KS533 were investigated. The host strain encodes the K1 capsule, and P and S fimbriae, but neither hemolysin nor the cytotoxic-necrotic factor CNF1 which suggests that this strain does not have a bigger pathogenicity island. The position of the RepFIC replicon is the same as for plasmid F. Hence, ColV plasmids and F share a region spanning more than half the length of plasmid F. ColV plasmids are found only in E. coli strains.

Escherichia coli

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Stability by multimer resolution of pJHCMW1 is due to the Tn1331 resolvase and not to the Escherichia coli Xer system

Article Abstract:

The transposon Tn1331 contributes to the stability of the plasmid pJHCMW1 rather than the mwr sequence. The latter sequence is similar to the E. coli Xer system found in the ColE1 plasmid.

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