Use of multiplex terminal restriction fragment length polymorphism for rapid and simultaneous analysis of different components of the soil microbial community

Article Abstract:

A study developed a multiplex terminal restriction fragment length polymorphism (M-TRFLP) fingerprinting method and validated it for simultaneous analysis of the diversity and community structure of two or more microbial taxa. The terminal restriction fragment length polymorphism profiles obtained with PCR products is amplified individually and with multiplexed and pooled PCR products are found to be consistent with each other in terms of the number, position, and relative intensity of peaks.

United Kingdom, Environmental aspects, Bacterial growth, Soil microbiology, Polymerase chain reaction, Genetic polymorphisms

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CO2- and anaerobiosis-induced changes in physiology and gene expression of different Listeria monocytogenees strains

Article Abstract:

The food borne pathogen Listeria monocytogenes is identified by its capability to increase at refrigeration temperatures in areas that are highly concentrated with carbon dioxide (CO2). The Listeria monocytogenes LO28 that can tolerate acid reacted in the similar way to CO2 as to other anaerobic, but mildly acidic environment.

Industrial Gas Manufacturing, Industrial gases, Carbon Dioxide, Microbiology, Listeria monocytogenes, Pathogenic microorganisms, Properties

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