Use of potassium depletion to assess adaptation of ruminal bacteria to ionophores

Article Abstract:

The ruminal bacteria in ionophore-supplemented animals show higher potassium depletion than unadapted bacteria, indicating that ruminal bacteria are more resistant to ionophores than unadapted bacteria. Monensin supplementation decreases the acetate-to-propionate ratio by a small value, but increases the potassium depletion value eight times. Lasalocid has no effect on the acetate-to-propionate ratio, bacterial protein and pH. However, lasalocid supplementation increases the potassium depletion values of monensin and lasalocid two times.

Physiological aspects, Observations, Potassium (Chemical element), Potassium, Bacteriology, Rumen, Rumen microbiology, Monensin, Lasalocid

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Molecular beacons: trial of a fluorescence-based solution hybridization technique for ecological studies with ruminal bacteria

Article Abstract:

Three novel fluorescent probes were utilized for the analysis of competition experiments with Ruminococcus albus and Finbrobacter succinogenes. Membrane and solution hybridizations with the molecular beacons led to an 80% decease in fluorescence due to the hybridization of the molecular probes with a single mismatched pair. The secondary structure of the ribosomal ribonucleic acid in the hybridization assay also exhibited refolding after the denaturation process.

Methods, Analysis, Microbial ecology, Nucleic acid probes, Hybridization

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An rNA approach for assessing the role of obligate amino acid-fermenting bacteria in ruminal amino acid deamination

Article Abstract:

A study was conducted on the influence of obligate amino acid-fermenting bacteria in ruminal amino acid deamination. 16S rRNA probes were used since 16S rRNA can be used to differentiate bacteria sans in vitro culturing. Results showed that the feed additive monensin is not capable of entirely counteracting the wasteful amino acid deamination of obligate amino acid-fermenting ruminal bacteria.

Research, Usage, Biodegradation, Ribosomal RNA, Amino acids, Rumen fermentation

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