Use of transposon promoter-probe vectors in the metabolic engineering of the obligate methanotroph Methylomonas sp. strain 16a for enhanced [carbon.sub.40] carotenoid synthesis

Article Abstract:

Transposable elements are used to identify the chromosomal locations in the obligate methanotroph Methylomonas sp. strain 16a, which has supported the expression of genes involved in the synthesis of [C.sub.40] carotenoids canthaxanthin and astaxanthin. The analysis has shown how metabolic engineering of microorganisms can be increased by identifying integration sites within the chromosome of the host that permit optimal expression of the target genes.

Gene expression, Chromosomes

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Diversity of carotenoid synthesis gene clusters from environmental Enterobacteriaceae strains

Article Abstract:

Several new carotenoid synthesis gene clusters from environmental Enterobacteriaceae strains that produce zeaxanthin and derivatives of this compound are isolated from a variety of environmental samples. The expression of the crtEYIB genes with and without idi has confirmed the effect of increasing carotenoid titer by the type II idi gene linked with the carotenoid synthesis gene clusters.

Enterobacter, Enterobacteriaceae, Carotenoid metabolism

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Novel carotenoid oxidase involved in biosynthesis of 4,4'- diapolycopene dialdehyde

Article Abstract:

Identification of a novel gene (CrtNb) involved in conversion of 4,4'-diapolycopene to 4,4'-diapolycopene aldehyde is reported. However, data from crtNb and expression of crtNb in Escherichia coli indicated that crtNb is a novel carotenoid oxidase catalyzing oxidation of the terminal methyl group(s) of 4,4'-diaponeurosporene and 4,4'-diapolycopene to the corresponding terminal aldehyde.

Escherichia coli, Oxidases, Genetic research

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