Visualization and enumeration of bacteria carrying a specific gene sequence by in situ rolling circle amplification

Article Abstract:

Rolling circle amplification (RCA) technique is applied to in situ nucleic acid amplification (in situ RCA) to visualize and count single Escherichia coil cells carrying a specific gene sequence. This method can become a valuable tool for studying free DNA uptake, especially in nonculturable bacteria, by optimizing the cell permeabilization condition for in situ RCA.

Escherichia coli, Microbial genetics

---

rRNA sequence-based scanning electron microscopic detection of bacteria

Article Abstract:

A new scanning electron microscopic method was developed for gaining both phylogenetic and morphological information about target microbes using in situ hybridization with rRNA-targeted oligonucleotide probes. Target cells were hybridized with oligonucleotide probes after gold labeling.

Analytical Laboratory Instrument Manufacturing, Analytical instruments, Scanning Microscopy, Usage, Observations, Bacteria, In situ hybridization

---

Similar abstracts:

• Abstracts: Purification and characterization of an endoglucanase from Streptomyces lividans 66 and DNA sequence of the gene
• Abstracts: Separation of marine bacteria according to buoyant density by use of the density-dependent cell sorting method
• Abstracts: Production of antibacterial compounds and biofilm formation by Roseobacter species are influenced by culture conditions
• Abstracts: Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA
• Abstracts: Genetic characterization and transmission cycles of Cryptosporidium species isolated from humans in New Zealand

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.