When coupled to natural transformation in Acinetobacter sp. strain ADP1, PCR mutagenesis is made less random by mismatch repair

Article Abstract:

Transformation-facilitated random PCR mutagenesis is successfully applied to proteins involved in the catabolism and regulation of phytochemicals in the nutritionally versatile Acinetobacter sp. strain. Random PCR mutagenesis is a powerful tool for structure-function analysis of targeted proteins, especially when coupled with DNA integration through natural transformation followed by selection for loss of function.

Analysis, Genetic aspects, DNA repair, Escherichia coli, Mutagenesis

---

Toxicity caused by hydroxycinnamoyl-coezyme a thioester accumulation in mutants of acinetobacter sp. strain ADP1

Article Abstract:

A key step in the hca-encoded pathway is activation of the natural substrates caffeate, p-coumarate, and ferulate by an acyl:coenzyme A ligase encoded by hcaC. An hcaC mutation from one isolate is characterized and is found to result in the substitution of an amino acid that is conserved in a functionally characterized homolog of HcaC.

Microbiology, Microbial mutation, Aromatic compounds, Acetyl coenzyme A

---

Similar abstracts:

• Abstracts: Influence of temperature and substrate concentration on bacterial growth yield in Seine River water batch cultures
• Abstracts: The cyro-EM structure of a translation initiation complex from Escherichia coli. Nascent ribosomes
• Abstracts: Attachment of and biofilm formation by Enterobacter sakazakii on stainless steel and enteral feeding tubes
• Abstracts: Detection and quantification of Wallemia sebi in Aerosols by real-time PCR, conventional PCR, and cultivation

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.