X-ray photoelectron spectroscopy and biochemical analysis of the surface of Lactobacillus helveticus ATCC 12046

Article Abstract:

A study of the chemical composition of the cell wall of Lactobacillus helveticus ATCC 12046 by comparing whole cells with surface-layer (S layer) stripped-cells using X-ray photoelectron spectroscopy reveals that peptidoglycans, lipoteichoic acids and polysaccharides constitute the intermediate layer of L. helveticus cell wall. The regular organization of the S-layer protein is interspersed with projections of non-proteinaceous elements, and the extracted S-layer comprises mainly of the pure protein. Homogeneous binding of ferritin to the intact cell surface suggests the presence of highly negatively charged areas near the S-layer.

Bacterial cell walls

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Rhapidosomes from Aquaspirillum itersonii: disruption and subsequent renaturation and characterization of the component proteins

Article Abstract:

Rhapidosomes of Aquaspirillum itersonii treated with 0.1 molar HCl and 6 molar concentration of GHCl break into protein components. The proteins formed by HCl treatment are in the form of secondary structures while that formed by GHCl treatment are in the form of a complex. On renaturing, these proteins form two high-molecular-weight complexes of molecular masses of 590 kDa and over 2500 kDa corresponding to aggregates of 6 and 36 dimers respectively. Rhapidosomes are protein compounds with nanometer scale tubular structure which are very stable and capable of self assembly.

Usage, Bacteria, Biomedical materials, Biomaterials

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Two-component signal-transducing systems involved in stress responses and vancomycin susceptibility in Lactobacillus sakei

Article Abstract:

PCR was used to amplify fragments of five rrp genes encoding Lactobacillus sakei response regulators (RRs), part of a loci that also includes hpd genes encoding histidine protein kinases (HPKs). The putative RRs were part of the OmpR-PhoB subclass of response regulators and the putative HPKs are members of the ENvZ-NarX orthodox histidine protein kinase family. RRs were implicated in susceptibility to the glycopeptide antibiotic vancomycin.

Observations, Cellular signal transduction

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