A dominant lethal genetic system for autocidal control of the Mediterranean fruitfly

Article Abstract:

The construction of strains of the Mediterranean fruit fly harboring a tetracycline-repressible transactivator (tTA) that causes lethality in early developmental stages of the parental transgenic tTA insects is described. Repressible dominant lethality potentially provides a replacement or redundant back-up for radiation-sterilization, the requirement for a dietary additive for larval viability would give a fail-safe aspect that would mitigate any failure in the irradiation procedure.

Pharmaceutical Preparation Manufacturing, Pharmaceutical preparations, Tetracycline Preparations, Genetically modified animals, Fruit-flies, Tetracycline, Tetracyclines, Fruit flies

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Female-specific insect lethality engineered using alternative splicing

Article Abstract:

The sex-specific alternative splicing in insects is used to engineer female-specific autocidal genetic systems in the Mediterranean fruit fly, Ceratitis capitata. The results have shown the potential of controlling gene expression through alternative splicing, which is used to confer female specificity on a conditional promoter.

Methods, Gene expression, Fly control

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Transposon-free insertions for insect genetic engineering

Article Abstract:

A method for post-integration elimination of all transposon sequences in the pest insect Medfly, Ceratitis capitata is described. Results show that insertions lack transposon sequences and are therefore impervious to transposase activity.

Transposons, Mediterranean fruit-fly, Mediterranean fruit fly

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