Activation of the mammalian immune system by siRNAs

Article Abstract:

The inhibition of gene expression through ribonucleic acid interference (RNAi) is a promising new experimental tool for gene function and target validation studies, though there are some unanticipated, different nonspecific effects associated with its use in mammals that raise concerns about the safe use of RNAi in vivo. A study describes such nonspecific activation of the immune system by small interfering RNAs (siRNAs) and suggests future directions that might allow the use of RNAi in vivo by bypassing or controlling these unwanted effects.

Genetic aspects, Immune system

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Lack of interferon response in animals to naked siRNAs

Article Abstract:

The possibility of administering naked, synthetic small interfering ribonucleic acids (siRNAs) to mice is presented by downregulating an endogenous or exogenous target without inducing an interferon response. The results indicated that the synthetic siRNAs were well tolerated in mice and the siRNA injections through the tail vein using high pressure were sufficient to downregulate an endogenous target gene in mice.

Mice as laboratory animals, House mouse

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Dicing with siRNA

Article Abstract:

Studies suggest that careful selection of short interfering RNA (siRNA) molecules that are effective at near sub-nanomolar levels could reduce non-specific simulation or repression of gene expression. Dicer is well characterized as an end recognition endonuclease capable of processing long double-stranded RNA (dsRNA) into siRNA duplexes of ~21 nucleotides.

United States, RNA interference

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