Design of siRNAs producing unstructured guide-RNAs results in improved RNA interference efficiency
Article Abstract:
A study describes a strong inverse correlation between the degree of guide-ribonucleic acid (RNA) secondary structure formation and gene silencing by small interfering (si)RNA. The analysis of the effect of the secondary structures of guide-RNA sequences on RNA interference (RNAi) efficiency provides a basis for better understanding RNA silencing pathways and improving the design of siRNAs.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2005
User Contributions:
Comment about this article or add new information about this topic:
Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA
Article Abstract:
A study is conducted which reports that synthetic short interfering RNAs (siRNA) formulated in nonviral delivery vehicles can be potent inducers of interferons and inflammatory cytokines both in vivo in mice and in vitro in human blood. Putative immunostimulatory motifs have allowed the design of siRNAs that can mediate RNAi but induce minimal immune activation.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2005
User Contributions:
Comment about this article or add new information about this topic:
Systematic identification of sense-antisense transcripts in mammalian cells
Article Abstract:
An experimental strategy that allows systematic identification of endogenous mRNA with long complementary regions to other transcripts is developed and applied to human normal mammary epithelial and breast cancer cells. The experiment revealed that when the antisense RNAs are specifically targeted, their corresponding mRNA levels significantly altered.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2004
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Robson Rhodes: birth of a business counsellor. Preserved for performance
- Abstracts: Are credit ratings procyclical? Ratings versus market--based measures of default risk in portfolio governance
- Abstracts: Geographic scope, product diversification, and the corporate performance of Japanese firms. Alliance networks and firm performance: the impact of repeated partnerships
- Abstracts: Expression profiling using a hexamer-based universal microarray
- Abstracts: Unraveling protein interaction networks with near-optimal efficiency. Protein interaction maps on the fly