High-throughput identification of transcription start sites, conserved promoter motifs and predicted regulons

Article Abstract:

The transcriptional start sites of 769 genes, 53 of which are transcribed from multiple start sites were identified using 62-probe level datasets obtained with a custom designed Caulobacter crescentus microarray chip. The transcription start sites enables targeted searching for regulatory protein binding motifs and such 27 motifs were identified 17 of which share no similarity to the characterized motifs of other Caulobacter crescentus.

Physiological aspects, Genetic aspects, Caulobacter

---

Compact, universal DNA microarrays to comprehensively determine transcription-factor binding site specificities

Article Abstract:

A maximally compact, synthetic DNA sequence design that represents all possible DNA sequence variants of a given length k on a single, universal microarray is presented for protein binding microarray (PBM) experiments. The binding specificities over a full range of affinities for five transcription factors of differential structural classes from yeast, worm, mouse and human were determined using the microarrays.

Usage, DNA microarrays

---

Discovering DNA regulatory elements with bacteria

Article Abstract:

Lack of data on the deoxyribonucleic acid (DNA)-binding specificities of most transcription factors presents a major obstacle in understanding transcriptional regulatory networks. A modified bacterial one-hybrid system, which offers a low-tech alternative for determining the DNA-binding specificities of transcription factors, is described.

Gene expression, DNA-ligand interactions, DNA binding

Similar abstracts:

• Abstracts: Patient safety during medication administration: The influence of organizational and individual variables on unsafe work practices and medication errors
• Abstracts: A probability-based approach for high-throughput protein phosphorylation analysis and site localization. A common open representation of mass spectrometry data and its application to proteomics research
• Abstracts: Discussion of 'Standard-setting measurement issues and the relevance of research'. Disclosure and the cost of capital: what do we know?
• Abstracts: Dividend valuation, trading and transactions costs: the 1997 partial abolition of dividend tax credit repayments
• Abstracts: US bank industry consolidation continues at a slow pace. US bank industry consolidation continues. S&P credit market services view

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.