Generic expansion of the substrate spectrum of a DNA polymerase by directed evolution

Article Abstract:

A strategy to expand the substrate range of DNA polymerases that recognize their substrates with exceptionally high specificity, restricting the use of unnatural nucleotides and the applications they enable. Mutants of Taq DNA polymerase that not only extended mismatches, but had acquired a generic ability to process a diverse range of noncanonical substrates is obtained while maintaining high catalytic turnover, processivity and fidelity.

All Other Miscellaneous Chemical Product and Preparation Manufacturing, Catalysts, Chemical preparations, not elsewhere classified, Analysis, DNA polymerases

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Molecular breeding of polymerases for amplification of ancient DNA

Article Abstract:

A strategy is described for the recovery of genetic information from damaged DNA. The results have shown that engineered polymerases can expand the recovery of genetic information from Pleistocene specimens and might benefit genetic analysis in paleontology, archeology and forensic medicine.

United States, Methods, Genetic aspects, Genetic research, Mammals, Fossil, Fossil mammals

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Molecular breeding of polymerases for amplification of ancient DNA

Article Abstract:

A strategy is described for the recovery of genetic information from damaged DNA. The results have shown that engineered polymerases can expand the recovery of genetic information from Pleistocene specimens and might benefit genetic analysis in paleontology, archeology and forensic medicine.

Research and Development in the Physical, Engineering, and Life Sciences, Paleontology, Usage, DNA damage, Polymerase chain reaction, Genetic code

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