No longer lost in translation

Article Abstract:

Two in vivo real-time single molecule assays have shown that stochastic gene expression results in punctuated bursts of protein synthesis. The results have clarified the origins of fluctuations in gene expression and these methods are applicable to unfolding the complexities of gene networks and elucidating mechanisms of pathological gene expression.

Gene expression, Genetic translation, Translation (Genetics)

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Climbing the protein ladder

Article Abstract:

A protein undergoes controlled acid digestion and creates two ladders on consisting of peptides containing N terminus and other containing C terminus. Analysis of the N terminal ladder of insulin by mass analysis of peptides is mentioned.

Analysis, Peptides, Insulin

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Protein sequencing by mass analysis of polypeptide ladders after controlled protein hydrolysis

Article Abstract:

Current techniques for determining protein modifications are not efficient. An approach for sequencing proteins and determining modifications with high speed, sensitivity and specificity is reported.

Polypeptides

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