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A tethered catalysis, two-hybrid system to identify protein-protein interactions requiring post-translational modifications

Article Abstract:

The yeast two-hybrid system is altered to make possible the detection of protein-protein interactions that necessitates a particular post-translational modification. In spite of the existence of the antagonizing enzyme activities, the catalysis still sustains the alterations made to the substrate.

Author: Fields, Stanley, Guo, Dawei, Hazbun, Tony R., Xu, Xin-Jing, Ng, Sze-Ling, Kuo, Min-Hao
Publisher: Nature Publishing Co.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2004
United States, Methods, Causes of, Protein binding, Yeast fungi, Yeasts (Fungi), Post-translational modification

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Protein-protein interaction networks and biology-what's the connection?

Article Abstract:

The problems associated with data handling protocols and biases in protein-protein interaction (PPI) networks analysis are presented. Solutions to ensure that network analysis and biological understanding remain connected are provided.

Author: Robertson, David L., Hakes, Luke, Pinney, John W., Lovell, Simon C.
Publisher: Nature Publishing Co.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2008
Proteins, Proteomics, Structure, Chemical properties

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