Extracellular accumulation of recombinant proteins fused to the carrier protein YebF in Escherichia coli

Article Abstract:

The study of the growth of commonly used laboratory strains of Escherichia coli revealed that they secrete YebF, a small soluble endogenous protein into the medium, which challenges the prevailing view that laboratory strain do not secrete proteins to the medium. The study demonstrates the efficient secretion of 'passenger' proteins linked to the carboxyl end of YebF.

United States, Physiological aspects, Genetic aspects, Escherichia coli, Nucleotide sequence, Base sequence, Bacterial proteins

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The site-specific incorporation of p-iodo-L-phenylalanine into proteins for structure determination

Article Abstract:

An attempt is made to show that the technology that makes it possible to genetically encode unnatural amino acids can be used to efficiently and site-specifically incorporate p-iodo-L-phenylalanine (iodoPhe) into proteins in response to an amber TAG codon.

Proteins, Amino acids, Phenylalanine, Chemical properties

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