Reversal of mouse hepatic failure using an implanted liver-assist device containing ES cell-derived hepatocytes

Article Abstract:

Mouse embryonic stem (ES) cells are differentiated into hepatocytes by coculture with a combination of human liver nonparenchymal cell lines and fibroblast growth factor-2, human activin A and hepatocyte growth factor to generate an alternative source of cells for bioartificial liver support. This kind of differentiation provides a foundation for differentiation of functional hepatocytes from human ES cells and a practical design for a clinically useful liver-assist device.

Physiological aspects, Mice, Liver cells, Hepatocytes, House mouse, Stem cell research

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A human beta-cell line for transplantation therapy to control type 1 diabetes

Article Abstract:

A reversibly immortalized human beta-cell clone (NAKT-15) is established by transfection of primary human beta-cells with a retroviral vector containing simian virus 40 large T-antigen (SV40T) and human telomerase reverse transcriptase (hTERT) cDNA flanked by paired IoxP recombination targets. Transplantation of NAKT-15 cells into streptozotocin-induced diabetic severe combined immunodeficiency mice resulted in perfect control of blood glucose within 2 weeks.

Care and treatment, Transplantation immunology

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Islets for all?

Article Abstract:

A report on the application of a reversible immortalization strategy for expanding human islets cells with retention of function is presented. Immortalized cell lines are generated by transformation of human islet cells with retroviral vectors expressing SV40 large T-antigen and human telomerase reverse transcriptase (TERT), with other of these genes flanked by loxP sites for later Cre recombinase-based excision.

Genetic aspects, Islet cell stimulating antibodies

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