Assignments of the Pfr-Pr FTIR difference spectrum of cyanobacterial phytochrome Cph1 using (super 15)N and (super 13)C isotopically labeled phycocyanobilin chromophore

Article Abstract:

The reversible red and far-red light-induced transitions of cyanobacterial phytochrome Cph1 from synechocystis PCC 6803 were investigated by Fourier transform infrared (FTIR) difference spectroscopy. High-quality light induced Pfr-Pr difference FTIR spectra were recorded for the 58 kDa N-terminal domain of Cph1 by repetitive photochemical cycling and signal averaging and the analysis of the effects of (super 13)C and (super 15)N isotope substitutions at selected sites in the phycocyanobilin chromophore by (super 15)N global labeling of the protein was carried.

Usage, Genetic aspects, Cyanobacteria, Fourier transform infrared spectroscopy

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Relative ground and excited-state p[K.sub.a] values of phytochromobilin in the photoactivation of phytochrome: a computational study

Article Abstract:

An analysis presents a computational study of the relative ground and excited-state p[K.sub.a] values of different pyrrole moieties of phytochromobilin in the photoactivation of phytochrome. The analysis proves that light absorption and isomerization have no effect on the acidity of the compound.

Science & research, Research, Optical properties, Isomerization, X-ray analysis, Absorption of light, Light absorption

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Dipolar relaxation within the protein matrix of the green fluorescent protein: a red edge excitation shift study

Article Abstract:

The solvent dynamics around the fluorophore in enhanced green fluorescent protein (EGFP) under various conditions is monitored by employing the wavelength-selective fluorescence method. The results suggest that EGFP exhibit a progressive red edge excitation shift (REES) in buffer and glycerol.

Electric properties, Bacterial proteins, Dielectric relaxation, Wavelength

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