Excess enthalpies of mixing in phospholipid-additive membranes

Article Abstract:

Research was conducted to examine the excess enthalpies of mixing in phospholipid-additive membranes. The objective of the study is to provide a general approach to evaluate nonideal mixing heats obtained by isothermal titration calorimetry in terms of an excess enthalpy function. Results demonstrate that the transfer heats of the detergent or the lipid to the mixed membranes may differ from the excess enthalpies, showing the major importance of this differentiation for a proper interpretation of the experimental results.

Research, Usage, Phospholipids, Lipid membranes, Enthalpy, Calorimeters

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Interactions in oligonucleotide hybrid duplexes on microarrays

Article Abstract:

Affmetrix GeneChip intensity data was investigated in terms of chip-averaged sensitivities over all perfect match (PM) and mismatch (MM) probes possessing a common triple of neighboring bases in the middle of their sequence. The results provided a set of molecular nearest-neighbour and single base related interaction parameters, which considered specific properties of duplex formation in microarray hybridization experiments.

Biological Product (except Diagnostic) Manufacturing, Drugs, DNA Probes, DNA microarrays

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Sensitivity of microarray oligonucleotide probes: Variability and effect of base composition

Article Abstract:

Improved understanding and predictability of oligonucleotide hybridization behavior is required for the optimization of probe design and analysis algorithms for microarray experiments. Physiochemical theory of GeneChip probe sensitivities divided the probe intensity into an averaged intensity value that served as a relative measure of the RNA target concentration and the sensitivity of each probe.

Nucleotide sequencing

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