Heightened electromagnetic fields between metal nanoparticles: surface enhanced Raman scattering from metal-cytochrome c-metal sandwiches

Article Abstract:

A study was conducted to examine interparticle electromagnetic coupling in nanoparticle:molecule:nanoparticle sandwiches. The sandwiches were prepared by adsorption of cytochrome-coated colloidal gold nanoparticles onto aggregated colloidal silver. Affinity chromatography was carried out to purify horse heart cytochrome c while silver sols were prepared by aqueous reduction of AgNO3 with Na3-citrate. Results indicated the presence of heightened electromagnetic fields between metal nanoparticles.

Nanotechnology, Molecular dynamics, Electromagnetism

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Protein:colloid conjugates for surface enhanced Raman scattering: stability and control of protein orientation

Article Abstract:

A study was conducted to examine conjugates between cytochrome c and colloidal silver particles that support technique of positioning a biomolecule analyte between two metal surfaces. Silver sols were obtained through the aqueous reduction of AgNO3 with trisodium citrate. Results indicated that Cc:au conjugate adsorbed to aggregates of colloidal silver supported greater stability to conformational and spin-state changes than Cc directly adsorbed to silver aggregates.

Analysis, Proteins, Colloids, Cytochrome c, Silver compounds, Biomolecules, Raman effect

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Barcoded metal nanowires: optical reflectivity and patterned fluorescence

Article Abstract:

The wavelength-dependent optical reflectivity of individual stripes in metallic barcodes is reported and the way wavelength-dependence impacts the intensity of fluorescence from sandwich immuno- and hybridization assays performed on the particle surface, is analyzed. The reflectivity for a variety of metals incorporated as 1 to 2 micrometer stripes in barcoded nanowires is described and fluorescence intensity as a function of underlying metal identity.

Science & research, Fluorescence, Optical properties, Chemical properties, Reflection (Optics), Reflection (Physics)

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