Origin of the anomalous two-photon absorption in fluorescent protein DsRed

Article Abstract:

The red fluorescent protein DsRed has exhibited a two-photon excitation band around 760 nm, which is not accompanied by any feature in the corresponding one-photon spectral region. Time-dependent density functional theory (DFT) is used to explain the effect, as arising from an electronic excitation of the DsRed chromophore with ability to couple with a charge-transfer state, through an effective two-photon absorption channel.

Science & research, Research, Usage, Binding proteins

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Predictions of novel two-photon absorption bands in fluorescent proteins

Article Abstract:

The two-photon absorption bands for the lowest excitations in various fluorescent proteins are measured. These proteins are found to exhibit multiphoton excitation and hence can be used in bioimaging.

Mechanical properties

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Resonance enhancement of two-photon absorption in fluorescent proteins

Article Abstract:

The phenomenon and the resonance enhancement of two-photon absorption (2PA) in fluorescent proteins are discussed.

Observations, Confocal microscopy

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