Probing folded and unfolded states of outer membrane protein A with steady-state and time-resolved tryptophan fluorescence

Article Abstract:

Folded and unfolded states of Escherichia coli outer-membrane protein A (OmpA) were probed with steady-state and time resolved tryptophan (Trp) fluorescence. The findings show that the Trp environments of OmpA folded in micelles and phospholipid bilayers are different and Trp mobility is reduced when OmpA is folded in micelle and even lower mobility when the protein is folded in a bilayer.

Escherichia coli, Membrane proteins, Phospholipids, Tryptophan metabolism

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Resonance Raman spectroscopy of Met121Glu azurin

Article Abstract:

The resonance Raman spectra from pseudomonas aeruginosa Met121Glu azurin is measured at wavelengths throughout the 600-nm absorption band at low and high pH. Analysis of the 600-nm absorption band and resulting resonance Raman excitation profiles under both pH conditions indicated that the excited-state distortions in this mutant are smaller than those in the native protein.

Usage, Pseudomonas aeruginosa, Optical properties, Thermal properties, Chemistry, Physical and theoretical, Physical chemistry, Raman spectroscopy

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Dynamic ionic strength effects in fast bimolecular electron transfer between a redox metalloprotein of high electrostatic charge and an inorganic reaction partner

Article Abstract:

The ionic strength dependence of the bimolecular electron transfer reaction between clostridium pasteurianum rubredoxin(III) and photochemically generated [Ru(bpy)3](super +) is investigated. The variation in ET rate constant over the 0.005-2.0 M ionic strength range is quantitatively consistent with the high negative charge and size of rubredoxin(III).

Clostridium, Electron transport, Photochemistry

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