Ring-polymer dynamics in gels: supercoiled and relaxed circular DNA in polyacrylamide

Article Abstract:

The electrophoretic behavior of circular DNA in polyacrylamide gels at low and intermediate fields has been studied. Two sizes of linear DNA are used as controls in the research. One has the same contour length and the other has the same radius-of-gyration as the circles. It will be demonstrated that the behavior of the circles is different from linear molecules but not because of impalement. Results indicate that linear dichroism (LD) can be useful in studying trapped DNA. LD has the capability to resolve the three types of migration in spite of being present together at the same time.

Circular DNA, Acrylamide

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Dynamics of oligonucleotides adsorbed on thermonsensitive core-shell latex particles

Article Abstract:

The whole motion of the oligonucleotides(ODNs) and the randomization of their orientations were severely limited by the collapse of the PNIPAM chains. Fluorescence anisotropy was found to be adequate tool to follow the dynamics of adsorbed oligonucleotides on the PNIPAM shell along the volume phase transition.

Biological Product (except Diagnostic) Manufacturing, Drugs, Thymine, Chemistry, Physical and theoretical, Physical chemistry

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Ultrafast excited-state dynamics of oxazole yellow DNA intercalators

Article Abstract:

The excited-state dynamics of the DNA intercalator YO-PRO-1 and of three derivatives is examined in water and in DNA by using ultrafast fluorescence spectroscopy. The decay of the fluorescence polarization anisotropy has shown ultrafast hopping of the excitation energy between the intercalated dyes.

Science & research, Optical properties, Fluorescence spectroscopy, Structure, Oxazoles, DNA synthesis

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