Separating structural heterogeneities from stochastic variations in fluorescence resonance energy transfer distributions via photon distribution analysis

Article Abstract:

A probability distribution analysis (PDA) method is established that quantitatively and precisely describes the shapes of fluorescence resonance energy transfer (FRET) signal distributions measured by two-color photon counting techniques. Inclusion of the effects of background and stochastic processes allow the prediction of shot-noise-limited shapes of histograms generated from single-photon counting data with high precision.

Analysis, Stochastic analysis, Photons, Energy transformation

---

Probability distribution analysis of single-molecule fluorescence anisotropy and resonance energy transfer

Article Abstract:

The probability distribution analysis (PDA) method which could be used to predict the shape of anisotropy histograms for any given expected ensemble anisotropy, signal intensity distribution and background is described. This method can be used as a sensitive tool to detect biomolecular heterogeneities in space and time.

Usage, Anisotropy

---

Photochromicity and fluorescence lifetimes of green fluorescent protein

Article Abstract:

It appears that wild-type green fluorescent protein of the bioluminescent jellyfish Aequorea and its mutants clearly shows multiexponential fluorescence decays at all excitation wavelengths. The photochromicity effect does not change the fluorescence lifetimes, but only the pre-exponential factors.

Research, Physiological aspects, Jellyfishes

Similar abstracts:

• Abstracts: Protein structure and dynamics from single-molecule fluorescence resonance energy transfer. Energy transfer in unsymmetrical phenylene ethynylene dendrimers
• Abstracts: Comparative study on solvation free energy expression in reference interaction site model integral equation theory
• Abstracts: "Reduced" distributed monopole model for the efficient prediction of energy transfer in condensed phases. Free energy and structure of dislocation cores in two-dimensional crystals
• Abstracts: Understanding the structural properties of a dendrimeric material directly from powder X-ray diffraction data
• Abstracts: Designs of Gd(III)-based magnetic resonance imaging contrast agents: Static and transient zero-field splitting contributions to the electronic relaxation and their impact on relaxitivity

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.