Purification and partial characterization of cellular retinol-binding protein, type two, from human small intestine

Article Abstract:

Human cellular retinol-binding protein, type II /hCRBP(II)A and hCRBP(II)B~ was purified from human small intestine homogenates. Amino acid sequence analysis revealed that of the 36 residues in the N-terminal of hCRBP(II)A, only one differs from the rat cellular retinol-binding protein type IIA. Although hCPRB(II)B was blocked at the N-terminal, this blockage had no effect in its binding properties. Competitive binding assay revealed that hCPRB(II) binds both retinal and retinol. Immunohistochemical analysis of the jejunum showed that hCPRB(II) has higher densities in the jejunum's proximal end.

Carrier proteins, Transport proteins, Intestinal absorption, Jejunum

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Sodium-dependent transport of riboflavin in brush border membrane vesicles of rat small intestine is an electrogenic process

Article Abstract:

A study was done on the process which initiates sodium-dependent transport of riboflavin in brush border membrane vesicles of rat small intestines. Results show that riboflavin transport across the intestinal brush border membrane is an electrogenic process which is aided by a specific sodium ion riboflavin cotransport system at physiological substrate concentrations. The process is affected by changes in pH which is reflective of its electrogenic nature.

Rats, Intestine, Small, Small intestine, Brush border membrane, Vitamin B2, Riboflavin

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Inanition may reduce alkaline phosphatase activity in liver and intestine of zinc-deficient mice

Article Abstract:

A study was done on the effects of inanition on alkaline phosphatase deficiency in liver and intestine of zinc-deficient mice. Results show that feeding mice with a basal diet containing three percent lipid improves inanition in zinc-deficient mice. It was further shown that liver alkaline phosphate activity decreases as a response to associated inanition in mice and not to the deficiency of zinc.

Research, Zinc in the body, Zinc (Nutrient), Alkaline phosphatase

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