Mammalian heparanase: gene cloning, expression and function in tumor progression and metastasis

Article Abstract:

Mammalian heparanase is discussed relative to gene cloning, function and expression in tumor metastasis and progression. A 50-kDa heparanse from human hepatoma and placenta was purified. Cloning of the cDNA and gene encoding the enzyme is reported. The study discussed involved what is apparently the first cloned mammalian heparan sulfate (HS)-degrading enzyme and gives direct evidence of its role in tumor metastasis. Overexpression of the heparanse cDNA in low- or nonmetastatic tumor cells brought high metastatic potential in experimental mice.

Israel, Genetic aspects, Mice, Mice (Rodents), Cloning, Proteoglycans

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Cloning of mammalian heparanase, an important enzyme in tumor invasion and metastasis

Article Abstract:

Cloning of mammalian heparanase is discussed. It is an endoglycosidase important in tumor invasion and metastasis and important in the degradation of the extracellular matrix by invading cells, notably metastatic tumor cells and migrating leukocytes. The cDNA sequence of the human platelet enzyme is reported. Identification of highly homologous sequences in activated mouse T cells and in a highly metastatic rat adenocarcinoma has been carried out.

Australia, Cytochemistry, Rats, Extracellular matrix, Physiology, Glycosaminoglycans, Mucopolysaccharides

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Heparanase: Breaking down barriers in tumors

Article Abstract:

Heparanase, long-sought, has been cloned and characterized functionally adding to understanding and potential manipulation of metastasis and inflammatory processes. More is known about autoimmune conditions, as well. It has taken 20 years to isolate a heparanase gene, mainly because the enzymes are very unstable and it is difficult to design specific quantitative assays.

United States

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