Rapid detection of mutagens by induction of luciferase-bearing prophage in Escherichia coli

Article Abstract:

A new approach to mutagenicity testing in Escherichia coli is discussed. The method involves induction of a luciferase-containing prophage by rapid screening assays. The mutagens are detected based on the bioluminescence of the prophage induction. The assay detected three mutagens: ethyl methanesulfonate, methyl methanesulfonate and mitomycin C. Since mutagenicity in bacteria is correlated with carcinogenicity in humans, the results of mutagenicity testing may provide information on the potential effects of the mutagens to humans.

Methods, Escherichia coli, Bacteriophages, Spectra

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Formation of mutagens from the photooxidations of 2-4-ring PAH

Article Abstract:

A new method for forming mutagenic derivatives reacts a series of 2-4-ring polycyclic aromatic hydrocarbons (PAH) in an environmental chamber until the reactants are ready for fractionation. Extracts are separated from effluents using high-performance liquid chromatography and tested for mutagenicity using modified Ames Salmonella bioassay. Gas chromatography or mass spectrometry can be used to detect PAH and divide it according to the number of its mutagenic products.

Polycyclic aromatic hydrocarbons

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