Binding of brassinosteroids to the extracellular domain of plant receptor kinase BRI1

Article Abstract:

The first evidence for direct binding of active brassinosteroids to BRI1, a leucine-rich repeat (LRR) receptor kinase localized to the plasma membrane, using a biotin-tagged photoaffinity castasterone (BPCS), a biosynthetic precursor of brassinolide, is presented. The results show that brassinosteroids bind directly to the 94 amino acids comprising island domain (ID)-LRR22 in the extracellular domain of BRI1, and define a new building domain for steroid hormones.

Protein binding, Protein kinases, Steroids (Organic compounds), Biotin, Steroids

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Regulation of ethylene gas biosynthesis by the Arabidopsis ET01 protein

Article Abstract:

Ethylene-overproducer mutation, ETO1, is a gene that negatively regulates ACS activity and ethylene production. It is proposed that, ETO1serves as a substrate-specific adaptor protein.

Cyclic Crude and Intermediate Manufacturing, Ethylene, Gene mutations, Gene mutation, Biosynthesis, Arabidopsis

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