Cell-free V(D)J recombination

Article Abstract:

Researchers have succeeded in reconstituting the entire V(D)J recombination reaction in a cell-free system. It was established that the RAG proteins are required in the later stages for proper joining of coding ends, as well as being involved in cleavage. This provides proof that cleavage and joining, the stages of V(D)J recombination, are closely connected. Cell-free V(D)J recombination has also assisted in distinguishing the various effects of cellular DNA ligases.

Genetic recombination

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Rag mutations reveal robust alternative end joining

Article Abstract:

The removal of certain portions of murine Rag proteins has disclosed robust alternative non-homologous end joining (NHEJ) activity in NHEJ-deficient cells and some alternative joining activity even in wild-type cells. A two-tier model is proposed in which the Rag proteins have collaborated with NHEJ factors to preserve genomic integrity during V(D)J recombination.

Science & research, Gene mutations, Gene mutation, Proteins, Recombinant proteins

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New guardians of the genome

Article Abstract:

Non-homologous-end-joining (NHEJ) proteins have been identified as genome guardians. The NHEJ machinery seems to act as a caretaker, protecting against oncogenic DNA rearrangements.

DNA repair, Cancer, Cancer genetics

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