Direct observation of base-pair stepping by RNA polymerase

Article Abstract:

The development of an ultra-stable optical trapping system with angstrom-level resolution, which is used to monitor transcriptional elongation by single molecules of Escherichia coli RNA polymerase (RNAP) is presented. RNAP advances along DNA by a single base pair per nucleotide addition to the nascent RNA.

United States, Biological Product (except Diagnostic) Manufacturing, Nucleic Acid Derivatives, Escherichia coli, Nucleotides

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Structural basis for substrate loading in bacterial RNA polymerase

Article Abstract:

The crystal structures of the Thermus thermophilus is determined with non hydrolysable substrate analogue, adenosine-5'-[([alpha],[beta])-methyleno]- triphosphate (AMPcPP), and with AMPcPP and antibiotic streptolydigin at 3.0 angstrom resolution.

Bacterial genetics, Crystals, Crystal structure, Structure

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Backtracking by single RNA polymerase molecules observed at near- base- pair resolution

Article Abstract:

Backtracking and recovery by single molecules of RNAP is observed by combining ultrastable optical trapping apparatus with a novel two- bead assay to monitor transcriptional elongation with near- base- pair precision.

Enzymes, Other Basic Organic Chemical Manufacturing, RNA, Biomolecules, Chemical properties

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