Fast sequencing of oligosaccharides using arrays of enzymes
Article Abstract:
The reagent array analysis method (RAAM) is a new technique for sequencing oligosaccharides using exoglycosidase enzymes. Biochemists have been seeking a quick and convenient means of isolating the oligosaccharides due to their crucial physiological role in establishing the glycoconjugates' form and function. RAAM obtains the sequences by dividing an oligosaccharide solution into equal aliquots which are then incubated with an exactly defined exoglycosidase mixture. RAAM overcomes some of the shortcomings of existing methods such as being too time-consuming.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1992
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Protective effects of oligosaccharides in P-selectin-dependent lung injury
Article Abstract:
An investigation of the sialyl-Lewis X, a ligand for P-selectin, revealed that they reduced lung injury and decreased the tissue accumulation of neutrophils. Rats were injected with cobra venom which caused a neutrophil-dependent, oxygen radical mediatedand P-selectin-dependent lung injury. Results of the experiment indicate that sialyl-Lewis X carbohydrates can be used as a new method for anti-inflammatory therapy.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1993
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Oligosaccharide sequencing technology
Article Abstract:
Scientists are now able to ascertain the function and structure of N-glycans in very small quantities of glycoprotein using modern laboratory equipment. Methods used in N-glycan oligosaccharide analysis and predictive HPLC glycan profiling are given. A high performance liquid chromatography system with normal phase column and fluorescence detection capabilities is required for research of this kind.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1997
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